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分子细胞生物学实验教程电子书

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作       者:邹奕,周天鸿

出  版  社:暨南大学出版社

出版时间:2013-10-17

字       数:19.4万

所属分类: 科技 > 自然科学 > 生物科学

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Molecular cell biology involves an assortment of techniques, which are generally found difficult for undergraduate students. On reviewing courses taught by many other instructors of molecular cell biology, and giving the increasing number of overseas university students in China, it was apparent that there was a special need for the publication of a laboratory guideline in English for undergraduate lab exercises. The collection in this laboratory manual was carefully chosen to cover topics that were considered fundamental to an appreciation of modern molecular cell biology, and was modified to compromise the individual lab conditions in different Chinese institutions.
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Author's Comments

Chapter 1 Microscopy

1.1 Introduction: Light Microscope—Resolution vs Magnification

1.2 Bright Field, Dark Field, Phase Contrast

1.3 Fluorescence Microscopy and Confocal Microscopy

1.4 Electron Microscopy

1.5 Exercise: Use of a Bright Field Microscope

1.6 Exercise: Examination of Different Cell Types

Chapter 2 Subcellular Structures

2.1 Introduction: Cells, Organelles and Vital Staining

2.2 Exercise: Vacuole in Animal Cells

2.3 Exercise: Staining of Mitochondria in Human Cheek Epithelial Cells

2.4 Immuno-Staining and Fluorescent Labeling

2.4.1 Immuno-Staining

2.4.2 Fluorophores and Fluorescent Labeling

2.5 Exercise: Immunofluorescent Analysis of Microtubules

Chapter 3 Centrifugation and Subcellular Fractionation

3.1 Introduction: Equilibrium Density Gradient Centrifugation and Differential Sedimentation

3.2 Exercise: Purification of Nuclear and Mitochondria by Differential Sedimentation

3.3 Exercise: Assay of Fractions for Succinate Dehydrogenase (SDH)

Chapter 4 Cell Membrane and Cross-Membrane Transport

4.1 Introduction: Membranes and Its Properties

4.2 Exercise: Osmosis

4.3 Exercise: Hemolysis

4.4 Exercise: Pinocytosis

Chapter 5 Cell Cycle and Cell Division

5.1 Introduction: Types of Cell Division

5.2 Exercise: Study of Different Stages of Mitosis in Onion Root Tip Cells

5.3 Exercise: Study of Different Stages of Meiosis in Mouse Testis Cells

Chapter 6 Cytogenetics and Karyotyping

6.1 Introduction: Karyotyping

6.2 Exercise: Short Term Culture of White Blood Cells and Preparation of Metaphase Chromosomes

6.3 G-and C-Banding of Metaphase Chromosomes

6.3.1 Exercise: G-Banding of Metaphase Chromosomes

6.3.2 Exercise: C-Banding of Metaphase Chromosomes

6.4 Exercise: Karyotyping

Chapter 7 Cell Culture

7.1 Introduction: Principles of Animal Cell Culture

7.2 Sterilization Techniques

7.3 Exercise: Establishment of a Primary Culture

7.4 Exercise: Cell Viability Assay by Trypan Blue Exclusion

7.5 Exercise: MTT Assay for Cell Viability

7.6 Exercise: Mammalian Cell Transfection

7.6.1 Exercise: Lipofectamine Mediated Mammalian Cell Transfection

7.6.2 Exercise: Transfecting Mammalian Cells by Electroporation

7.7 Exercise: PEG Induced Fusion of Erythrocytes

Chapter 8 Apoptosis Analysis

8.1 Introduction: The Characteristic Cellular Changes of Apoptosis

8.2 Exercise: Induction of Apoptosis

8.3 Exercise: Vital Staining and Detection of Chromatin Condensation

8.4 Exercise: Detection of DNA Fragmentation

Chapter 9 Flow Cytometry

9.1 Introduction: Flow Cytometry and Signal Processing

9.2 Exercise: Cell Cycle Analysis by Propidium Iodide(PⅠ)Staining

9.3 Exercise: Cell Sorting by Fluorescence-Activated Cell Sorting (FACS)

Chapter 10 Cellular Immunology:Enzyme-Linked Immunosorbent Assay(ELISA)

10.1 Introduction: The History and the Application

10.2 Exercise: Direct ELISA(Also Known as Sandwich ELISA)

10.3 Exercise: Capture ELISA

Chapter 11 Molecular Cell Biology

11.1 Exercise: DNA Extraction and Purification

11.2 Exercise: Determination of DNA Concentration Using the Spectrophotometer

11.3 Exercise: Total RNA Isolation from Cells in Culture

11.4 Standard PCR

11.4.1 Introduction: Principles of PCR

11.4.2 Exercise: PCR Amplification of DNA

11.4.3 Exercise: Two-Step RT-PCR Amplification of β-Actin

11.5 Exercise: Southern Blot Using 32P Labeled dGTP

11.6 Measurement of Cell Protein Content

11.6.1 Introduction: Bradford Assay

11.6.2 Exercise: Protein Assay and Measurement of Protein Content of Cells (Level I)

11.7 Protein Extraction and Purification

11.7.1 Introduction: The Principles for Protein Extraction

11.7.2 Exercise: Whole Cell Extract Preparation

11.8 Exercise: Protein Electrophoresis

11.8.1 Introduction: Protein Electrophoresis and SDS-PAGE

11.8.2 Exercise: Protein Separation on SDS-PAGE

11.9 Protein Analysis and Detection

11.9.1 Exercise: Visualization of Proteins in Gels

11.9.2 Exercise: Semi-Dry Transfer of Protein onto Membrane

11.9.3 Exercise: Western Blot

Appendix: Chemical Preparations

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