分子细胞生物学实验教程电子书

Author's Comments

Chapter 1 Microscopy

1.1 Introduction: Light Microscope—Resolution vs Magnification

1.2 Bright Field, Dark Field, Phase Contrast

1.3 Fluorescence Microscopy and Confocal Microscopy

1.4 Electron Microscopy

1.5 Exercise: Use of a Bright Field Microscope

1.6 Exercise: Examination of Different Cell Types

Chapter 2 Subcellular Structures

2.1 Introduction: Cells, Organelles and Vital Staining

2.2 Exercise: Vacuole in Animal Cells

2.3 Exercise: Staining of Mitochondria in Human Cheek Epithelial Cells

2.4 Immuno-Staining and Fluorescent Labeling

2.4.1 Immuno-Staining

2.4.2 Fluorophores and Fluorescent Labeling

2.5 Exercise: Immunofluorescent Analysis of Microtubules

Chapter 3 Centrifugation and Subcellular Fractionation

3.1 Introduction: Equilibrium Density Gradient Centrifugation and Differential Sedimentation

3.2 Exercise: Purification of Nuclear and Mitochondria by Differential Sedimentation

3.3 Exercise: Assay of Fractions for Succinate Dehydrogenase （SDH）

Chapter 4 Cell Membrane and Cross-Membrane Transport

4.1 Introduction: Membranes and Its Properties

4.2 Exercise: Osmosis

4.3 Exercise: Hemolysis

4.4 Exercise: Pinocytosis

Chapter 5 Cell Cycle and Cell Division

5.1 Introduction: Types of Cell Division

5.2 Exercise: Study of Different Stages of Mitosis in Onion Root Tip Cells

5.3 Exercise: Study of Different Stages of Meiosis in Mouse Testis Cells

Chapter 6 Cytogenetics and Karyotyping

6.1 Introduction: Karyotyping

6.2 Exercise: Short Term Culture of White Blood Cells and Preparation of Metaphase Chromosomes

6.3 G-and C-Banding of Metaphase Chromosomes

6.3.1 Exercise: G-Banding of Metaphase Chromosomes

6.3.2 Exercise: C-Banding of Metaphase Chromosomes

6.4 Exercise: Karyotyping

Chapter 7 Cell Culture

7.1 Introduction: Principles of Animal Cell Culture

7.2 Sterilization Techniques

7.3 Exercise: Establishment of a Primary Culture

7.4 Exercise: Cell Viability Assay by Trypan Blue Exclusion

7.5 Exercise: MTT Assay for Cell Viability

7.6 Exercise: Mammalian Cell Transfection

7.6.1 Exercise: Lipofectamine Mediated Mammalian Cell Transfection

7.6.2 Exercise: Transfecting Mammalian Cells by Electroporation

7.7 Exercise: PEG Induced Fusion of Erythrocytes

Chapter 8 Apoptosis Analysis

8.1 Introduction: The Characteristic Cellular Changes of Apoptosis

8.2 Exercise: Induction of Apoptosis

8.3 Exercise: Vital Staining and Detection of Chromatin Condensation

8.4 Exercise: Detection of DNA Fragmentation

Chapter 9 Flow Cytometry

9.1 Introduction: Flow Cytometry and Signal Processing

9.2 Exercise: Cell Cycle Analysis by Propidium Iodide（PⅠ）Staining

9.3 Exercise: Cell Sorting by Fluorescence-Activated Cell Sorting （FACS）

Chapter 10 Cellular Immunology:Enzyme-Linked Immunosorbent Assay（ELISA）

10.1 Introduction: The History and the Application

10.2 Exercise: Direct ELISA（Also Known as Sandwich ELISA）

10.3 Exercise: Capture ELISA

Chapter 11 Molecular Cell Biology

11.1 Exercise: DNA Extraction and Purification

11.2 Exercise: Determination of DNA Concentration Using the Spectrophotometer

11.3 Exercise: Total RNA Isolation from Cells in Culture

11.4 Standard PCR

11.4.1 Introduction: Principles of PCR

11.4.2 Exercise: PCR Amplification of DNA

11.4.3 Exercise: Two-Step RT-PCR Amplification of β-Actin

11.5 Exercise: Southern Blot Using 32P Labeled dGTP

11.6 Measurement of Cell Protein Content

11.6.1 Introduction: Bradford Assay

11.6.2 Exercise: Protein Assay and Measurement of Protein Content of Cells （Level I）

11.7 Protein Extraction and Purification

11.7.1 Introduction: The Principles for Protein Extraction

11.7.2 Exercise: Whole Cell Extract Preparation

11.8 Exercise: Protein Electrophoresis

11.8.1 Introduction: Protein Electrophoresis and SDS-PAGE

11.8.2 Exercise: Protein Separation on SDS-PAGE

11.9 Protein Analysis and Detection

11.9.1 Exercise: Visualization of Proteins in Gels

11.9.2 Exercise: Semi-Dry Transfer of Protein onto Membrane

11.9.3 Exercise: Western Blot

Appendix: Chemical Preparations