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Chapter 1 Microscopy
1.1 Introduction: Light Microscope—Resolution vs Magnification
1.2 Bright Field, Dark Field, Phase Contrast
1.3 Fluorescence Microscopy and Confocal Microscopy
1.4 Electron Microscopy
1.5 Exercise: Use of a Bright Field Microscope
1.6 Exercise: Examination of Different Cell Types
Chapter 2 Subcellular Structures
2.1 Introduction: Cells, Organelles and Vital Staining
2.2 Exercise: Vacuole in Animal Cells
2.3 Exercise: Staining of Mitochondria in Human Cheek Epithelial Cells
2.4 Immuno-Staining and Fluorescent Labeling
2.4.1 Immuno-Staining
2.4.2 Fluorophores and Fluorescent Labeling
2.5 Exercise: Immunofluorescent Analysis of Microtubules
Chapter 3 Centrifugation and Subcellular Fractionation
3.1 Introduction: Equilibrium Density Gradient Centrifugation and Differential Sedimentation
3.2 Exercise: Purification of Nuclear and Mitochondria by Differential Sedimentation
3.3 Exercise: Assay of Fractions for Succinate Dehydrogenase （SDH）
Chapter 4 Cell Membrane and Cross-Membrane Transport
4.1 Introduction: Membranes and Its Properties
4.2 Exercise: Osmosis
4.3 Exercise: Hemolysis
4.4 Exercise: Pinocytosis
Chapter 5 Cell Cycle and Cell Division
5.1 Introduction: Types of Cell Division
5.2 Exercise: Study of Different Stages of Mitosis in Onion Root Tip Cells
5.3 Exercise: Study of Different Stages of Meiosis in Mouse Testis Cells
Chapter 6 Cytogenetics and Karyotyping
6.1 Introduction: Karyotyping
6.2 Exercise: Short Term Culture of White Blood Cells and Preparation of Metaphase Chromosomes
6.3 G-and C-Banding of Metaphase Chromosomes
6.3.1 Exercise: G-Banding of Metaphase Chromosomes
6.3.2 Exercise: C-Banding of Metaphase Chromosomes
6.4 Exercise: Karyotyping
Chapter 7 Cell Culture
7.1 Introduction: Principles of Animal Cell Culture
7.2 Sterilization Techniques
7.3 Exercise: Establishment of a Primary Culture
7.4 Exercise: Cell Viability Assay by Trypan Blue Exclusion
7.5 Exercise: MTT Assay for Cell Viability
7.6 Exercise: Mammalian Cell Transfection
7.6.1 Exercise: Lipofectamine Mediated Mammalian Cell Transfection
7.6.2 Exercise: Transfecting Mammalian Cells by Electroporation
7.7 Exercise: PEG Induced Fusion of Erythrocytes
Chapter 8 Apoptosis Analysis
8.1 Introduction: The Characteristic Cellular Changes of Apoptosis
8.2 Exercise: Induction of Apoptosis
8.3 Exercise: Vital Staining and Detection of Chromatin Condensation
8.4 Exercise: Detection of DNA Fragmentation
Chapter 9 Flow Cytometry
9.1 Introduction: Flow Cytometry and Signal Processing
9.2 Exercise: Cell Cycle Analysis by Propidium Iodide（PⅠ）Staining
9.3 Exercise: Cell Sorting by Fluorescence-Activated Cell Sorting （FACS）
Chapter 10 Cellular Immunology:Enzyme-Linked Immunosorbent Assay（ELISA）
10.1 Introduction: The History and the Application
10.2 Exercise: Direct ELISA（Also Known as Sandwich ELISA）
10.3 Exercise: Capture ELISA
Chapter 11 Molecular Cell Biology
11.1 Exercise: DNA Extraction and Purification
11.2 Exercise: Determination of DNA Concentration Using the Spectrophotometer
11.3 Exercise: Total RNA Isolation from Cells in Culture
11.4 Standard PCR
11.4.1 Introduction: Principles of PCR
11.4.2 Exercise: PCR Amplification of DNA
11.4.3 Exercise: Two-Step RT-PCR Amplification of β-Actin
11.5 Exercise: Southern Blot Using 32P Labeled dGTP
11.6 Measurement of Cell Protein Content
11.6.1 Introduction: Bradford Assay
11.6.2 Exercise: Protein Assay and Measurement of Protein Content of Cells （Level I）
11.7 Protein Extraction and Purification
11.7.1 Introduction: The Principles for Protein Extraction
11.7.2 Exercise: Whole Cell Extract Preparation
11.8 Exercise: Protein Electrophoresis
11.8.1 Introduction: Protein Electrophoresis and SDS-PAGE
11.8.2 Exercise: Protein Separation on SDS-PAGE
11.9 Protein Analysis and Detection
11.9.1 Exercise: Visualization of Proteins in Gels
11.9.2 Exercise: Semi-Dry Transfer of Protein onto Membrane
11.9.3 Exercise: Western Blot
Appendix: Chemical Preparations
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